Abstract: In recent years, most studies on the impact of habitat loss and biological invasion on species diversity have focused on a single factor. In fact, events such as habitat loss and biological invasion do not always occur independently, but may coexist and have a combined effect on species coexistence. This study focuses on a three-species competition system after biological invasion to explore the survival conditions of species under different invasion modes, and introduces the habitat loss process after invasion to clarify the impact mechanism of different habitat loss modes on the outcome of the invasion. It uses a spatially explicit cellular automaton simulation method to carry out the innovative research, fully considers statistical randomness and spatial relevance, and analyzes the simulation results. The results show that: When the loss process tends to be anthropogenic, such as the loss pattern of large farmland ponds and road villages, habitat loss will intensify the diffusion and competitive invasion of alien species. When the loss process tends to be naturally destructed, such as random loss or gradient pattern loss, habitat loss will inhibit the diffusion and competitive invasion of species, thus alleviating the harm of native species invasion to some extent. Finally, the effect of habitat loss on the outcome of competitive diffusion invasion is opposite to that of diffusion invasion and competitive invasion. The results of this study provide inspirations and theoretical guidance for the research and policy implementation of biological invasion control.