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Table of Content

    21 March 2023, Volume 37 Issue 2 Previous Issue    Next Issue
    “Research on State Estimation and Prediction Technology of Advanced Power Battery”Special Column

    Study on different data-driven models of the lithium battery capacity-fading model

    2023, 37 (2):  12-18.  doi: 10.3969/j.issn.1674-8425(z).2023.02.002
    Abstract ( 275 )   PDF (2015KB) ( 103 )   Save

    At present, the lithium-ion power battery system is the core component of new energy vehicles, and an effective battery management system can improve electrical performance and safety performance for the vehicles. It is worth mentioning that battery cycle life is an important indicator of power battery safety. Moreover, the most direct parameter to characterize battery performance degradation is the remaining capacity of a battery. Therefore, remaining capacity prediction of the battery after multiple charge-discharge cycles has become a research hotspot.

    In a normal working process, the residual capacity of power batteries cannot be measured by direct testing means, so it is necessary to use battery characteristic parameters that are easy to measure to predict the residual capacity. Many scholars at home and abroad extract characteristic values from dynamic charge-discharge curves, but this method is easily affected by battery operating conditions. In order to avoid the influence of dynamic conditions, voltage increase during the resting time of the battery after the discharge period is used as a health factor to characterize the decline of battery capacity. The mapping model between health factors and battery remaining capacity is established by using backward propagation (BP) algorithm and a support vector machine (SVM).

    The test data of more than 500 cycles of charge-discharge of an in-service 42 Ah ternary lithium battery are taken as samples. Among the samples, the first 400 cycles of the data are set as training samples. Then, through the subsequent 111 cycles of the data, this paper predicts the remaining capacity of the battery by using the model of the BP algorithm and the SVM respectively. The data results and the change trend of the test data in the two predicted models are the same. The MSE for the model of BP and SVM are 1.4% and 0.6% respectively.

    The experiment results show that, after multiple charge-discharge cycles, there is an obvious linear relationship between the voltage rise sequence during the shelving period and the maximum remaining capacity of the ternary lithium battery. As health factors, voltage rise parameters can be a theoretical reference for the analysis of the remaining capacity of the same type of ternary lithium batteries in practical application. The BP and SVM models can be used to accurately predict the remaining capacity of batteries. Compared with the BP algorithm, the SVM algorithm has better performance when used for small-scale data, with more accurate and effective prediction.

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    Sorting method of retired lithium-ion batteries considering dynamic curve characteristics

    2023, 37 (2):  19-27.  doi: 10.3969/j.issn.1674-8425(z).2023.02.003
    Abstract ( 175 )   PDF (2348KB) ( 79 )   Save
    Lithium-ion batteries have the advantages of high energy density, long cycle life and being free from memory effect, and are widely used in electric vehicles and energy storage. In recent years, how to deal with retired batteries has become an urgent development problem for the new energy vehicle industry. It is an effective way to solve this problem by applying retired batteries to the energy storage system on a large scale. When the retired lithium-ion batteries are grouped, the performance difference between individual batteries will cause a rapid attenuation of module performance, shortening the module cycle. When a lithium-ion battery is assembled from these multiple retired cells, the difference in the performance of each cell will cause a rapid attenuation of battery performance. The attenuation of battery performance will reduce cycle life and even cause safety problems. In order to reduce performance difference of decommissioned batteries after grouping and improve the clustering effect of K-means algorithm, this paper takes 100 of the decommissioned 18 650 lithium batteries purchased in the same batch as the research objects for charge-discharge tests and internal resistance tests. The charge and discharge experiments and internal resistance tests of 100 retired lithium-ion cells are conducted. A consistency sorting method is proposed based on dynamic curve. Firstly, in terms of parameter selection, considering that the internal resistance of a battery and the internal resistance of a connector will consume part of the electric during the charging and discharging process, and the electrochemical polarization and concentration polarization of Li+ insertion and detachment will also cause a part of energy loss, the difference between the charging energy and the discharging energy can be used to describe the chemical polarization and concentration polarization in the general testing process. A consistent sorting and reorganization method for retired lithium-ion batteries considering dynamic curve characteristics is proposed, and the multi-parameter sort is carried out by using six indicators, including capacity, energy difference, charging voltage, discharging voltage, charging resistance and discharging resistance. In consideration of the correlation between the sorting variables, in order to reduce the sorting variables and simplify the calculation, factor analysis is applied to sort variables of retired batteries, and the batteries are classified by using the inter-group connection clustering method and the square Euclidean distance as the measurement standard. In terms of sorting methods, the dynamic changes of battery parameters during charging and discharging are taken into account through the dynamic characteristic sorting method. Combining with multi-parameter sorting, higher consistency can be achieved. The existing dynamic sorting methods generally use voltage curve for sorting, but this method cannot reflect current, capacity and other performance parameters. The voltage curve during constant voltage charging cannot show the change trend of battery energy and capacity; the energy curve at the time of shelving cannot represent the change of battery voltage. In addition, the consistency of voltage and capacity should be taken into account during battery combination to avoid energy waste. Therefore, on the basis of multi-parameter sorting, energy difference is used to represent the difference of cell polarization. Then, the K-means algorithm is used to carry out dynamic curve sorting based on voltage deviation and capacity deviation. Finally, in terms of algorithm improvement, to solve the problem of an uncertain K value, the voltage and energy curves are normalized respectively. According to an analysis of frequency distribution histogram and frequency distribution curve, the K value of clusters is determined. The voltage standard deviation of 100 retired lithium-ion batteries before sorting is 0.043 1, of Class I batteries after sorting is 0.203 7, and of Class Ⅱ batteries after sorting is 0.011 1. It can be seen that the voltage consistency of type I battery is poor, indicating that performance degradation of the battery pack is caused by performance degradation of very few batteries. The results of experiment show that the method can effectively improve inconsistency of the battery. The consistency of charging voltage increases by about 60%~94%. The consistency of discharge voltage increases by about 10%~41%. The consistency of capacity increases by about 54%~67%.
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    “Research on Energy Management Technology of New Energy Vehicles” Special Column

    Design of an economical cruise speed planning and control method for pure electric vehicles

    2023, 37 (2):  38-49.  doi: 10.3969/j.issn.1674-8425(z).2023.02.005
    Abstract ( 296 )   PDF (3775KB) ( 220 )   Save
    In order to improve the driving range of pure electric vehicles, this paper proposes an economical cruise speed planning and control method. According to the driving environment of a vehicle, such as the following mode or the cruise mode, a short-term speed planning method based on long short-term memory (LSTM) and a long-term speed planning method based on genetic algorithm (GA) are proposed respectively. The economical cruise control system is designed based on a hierarchical structure, in which the upper layer obtains the planned speed profile by constructing the objective function of the problem to be optimized and solving it with GA, and the lower layer takes the optimal speed curve as the input and realizes distance control and speed control based on the tracking controllers. The pure electric vehicle model and the economical cruise control system are built on the joint simulation platform of CarSim and Simulink. The research results show that the introduction of speed planning can provide the vehicle with the optimal speed curve after energy optimization in multiple scenarios so as to reduce the energy consumption of electric vehicles and improve travel efficiency.
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    Research on rule-based energy management of plug-in hybrid electric vehicles based on P2.5 configuration

    2023, 37 (2):  50-59.  doi: 10.3969/j.issn.1674-8425(z).2023.02.006
    Abstract ( 175 )   PDF (3596KB) ( 73 )   Save
    With increasingly severe problems of energy consumption and environmental pollution, under the guidance of fuel consumption and emission regulations, how to further reduce fuel consumption has become the top priority of the development of the automobile industry. With a consideration of both power and economy, Plug-in Hybrid Electric Vehicles (PHEV) have lower fuel consumption than traditional fuel vehicles and have multiple power drive modes in their design. Compared with pure electric vehicles, they do not have the problem of endurance mileage anxiety, and become the focus of the current automobile industry. However, fuel economy of PHEV requires a careful consideration of various factors. How to improve fuel economy has been the focus and difficulty of the automotive industry. The PHEV based on the P2.5 hybrid system configuration integrates drive motors into a particular input shaft of transmission, with high drive and braking efficiency. It can drive directly through the clutch and transmission and work together with the engine. Compared with other hybrid models, the P2.5 configuration PHEV has higher integration, less space, a smooth connection between oil and electricity, higher drive and braking efficiency, and superior fuel economy. Therefore, this paper takes the P2.5 configuration PHEV as the research object and proposes a rule-based energy management strategy to improve fuel economy of the P2.5 configuration PHEV. First of all, the power system of PHEV with P2.5 configuration is analyzed, and, combined with the advantages of this configuration, it is divided into five working modes: pure electric driving mode, pure engine driving mode, light load charging mode, hybrid driving mode, and regenerative braking mode. Secondly, based on the idea of the logical threshold value of the state of charge (SOC) of the power battery, a multi-phase energy management control strategy of CD-CS is proposed. According to the change trajectory of the SOC value of the battery, the active phase of PHEV is divided into two phases,namely charge-depleting (CD) and charge-sustaining (CS), and corresponding rules are formulated according to the SOC value of the battery to switch the two modes. On the premise of meeting the power, the vehicle is operated to use up the battery energy as much as possible to reduce fuel consumption within the driving range. Finally, according to the idea of the forward simulation model, the driver model, engine model, drive motor model, power battery model, vehicle dynamics model and other vital components of PHEV are simulated under different working conditions in Matlab/Simulink software based on experimental data. The results show that the vehicle model established in this paper and the proposed rule-based energy management strategy are accurate and effective. Compared with the pure engine mode,fuel savings of 100 km under WLTC, NEDC, UDDS and HWFET conditions increases by 34.6%, 38.8%, 63.3%, and 30.9%respectively. The simulation results show that fuel savings under all conditions increase by more than 30%, with a maximum increase of 63.3%. This fully demonstrates that the rule-based energy management strategy can effectively improve fuel economy of the engine.
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    Multi-objective energy management strategy for hybrid electric vehicles with an incorporation of battery temperature control

    2023, 37 (2):  60-67.  doi: 10.3969/j.issn.1674-8425(z).2023.02.007
    Abstract ( 147 )   PDF (2026KB) ( 144 )   Save
    In this paper, a multi-objective optimization energy management strategy for a parallel hybrid electric vehicle is established to balance fuel economy and battery temperature rise effect. A vehicle dynamics model and a battery temperature rise model are also established. A multi-objective parameter optimization based on Pareto optimal solutions is built by selecting the control parameters of power components and several battery related parameters. Considering the temperature accumulation effect under high rate currents, the upper limit control strategy of a motor torque based on temperature rise feedback is developed, so that the current amplitude is actively controlled to realize battery temperature control. The analysis results indicate that frontier Pareto solutions obtained by multi-objective optimization have a good balance between both fuel economy and battery temperature rise response. The motor torque threshold control strategy effectively adjusts current amplitude under high speed conditions, thus achieving the limit of battery heat accumulation.
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    Research on the regenerative braking energy distribution strategy of hub driven electric vehicles

    2023, 37 (2):  68-76.  doi: 10.3969/j.issn.1674-8425(z).2023.02.008
    Abstract ( 173 )   PDF (2944KB) ( 198 )   Save
    With the maximum range of motor braking participation during braking stability as the target, this paper uses a braking feedback control strategy of four-wheel hub driven electric vehicles as the research content, and considers the influence of coordinated control of the anti-lock braking system and motor braking on the regenerative braking. Based on the ideal braking force distribution I curve, the front and rear axle braking force distribution limit control line is determined, and a braking torque of two bridges is designed according to the motor power, ensuring an optimal distribution strategy of intervention priority and maximum participation of the feedback braking system on a single axle. An energy feedback control model based on Matlab/Simulink is constructed to analyse and evaluate the feasibility of regenerative braking under UDDS operating conditions. The simulation data show that, under a single operating condition, 0.126 kW·h of energy is recovered by using the optimised control strategy, which is 7.7% higher than that in the conventional fixed-proportional strategy. Compared with the ordinary fixed-proportional control and the control scheme without regenerative braking, the proposed control strategy reduces energy consumption by 1.99% and 7.30% respectively under the UDDS cycle conditions, and the energy saving effect is 7.29%. The peak value of impact during vehicle regenerative braking is only 1.72 m·s-3, resulting in good braking comfort.
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    Experimental study on energy flow of a hybrid commercial vehicle

    2023, 37 (2):  77-85.  doi: 10.3969/j.issn.1674-8425(z).2023.02.009
    Abstract ( 190 )   PDF (3992KB) ( 178 )   Save
    In this paper, the vehicle energy flow test of a heavy hybrid commercial vehicle is carried out on the chassis dynamometer with an ambient chamber under the C-WTVC test cycle. Energy flow characteristics, driving modes, the power system and key component working status and efficiency of the vehicle are studied. The test results show that the energy used to drive the vehicle accounts for 30.99% of the total energy. Heat transfer loss of the radiator and the motor cooling loop accounts for 8.89% and 2.6% of the total energy respectively. The remainder loss accounts for 50.67% of the total energy which includes engine pump gas loss, friction loss, combustion loss and exhaust enthalpy increase. In the driving mode, pure electric drive, parallel drive, drive power generation and the energy recovery mode account for 9.1%, 29.56%, 18.51% and 23.61% of the total cycle time respectively. The recovery efficiency of braking energy is 81.95%. There are about 65% of the engine working points located below 200 g/(kW·h) areas, and the thermal efficiency of the engine is 36.81% during the whole test cycle. About 24% of the motor working points are located in areas with a mechanical efficiency exceeding 94%. About 53% of the motor working points are located in areas with a mechanical efficiency below 92%, which mainly appear when the motor is driven by the engine to generate electricity or recover energy. This study provides guidance for improving the energy efficiency of hybrid commercial electric vehicles, and the following research can be focused on reduction of engine remainder loss and optimization of engines and motor working points.
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    Research on PHEV energy management strategy optimized by weighted double-Q learning algorithm

    2023, 37 (2):  86-96.  doi: 10.3969/j.issn.1674-8425(z).2023.02.010
    Abstract ( 161 )   PDF (4421KB) ( 205 )   Save
    As the key direction of development in the automobile field, Plug-in hybrid electric vehicles (PHEV) are energy-saving, environmental friendly and free from anxieties of endurance mileage. However, the control strategy of PHEV is relatively complex, involving the energy distribution of multiple power sources. How to design an efficient and reliable energy management strategy has become a hot and difficult issue in PHEV research. In order to improve the fuel economy and vehicle performance of PHEV, a PHEV energy management control strategy based on weighted double-Q learning is proposed, and the weighted double-Q learning algorithm is used to solve the energy distribution of PHEV in this paper. Furthermore, a vehicle model is built and simulated in Matlab/Simulink to verify the effectiveness and reliability of the proposed strategy. The results show that, compared with the rule-based CD/CS strategy, the fuel economy of the proposed strategy improves by 6.38% on average under different driving conditions. The fuel economy of the weighted double-Q learning strategy can reach 98% of that of the stochastic dynamic programming strategy under different driving conditions. The above results verify that the proposed strategy has good fuel economy and adaptability to different driving conditions.
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    Temperature rise characteristics of a permanent magnet synchronous motor under oil-water compound cooling

    2023, 37 (2):  97-103.  doi: 10.3969/j.issn.1674-8425(z).2023.02.011
    Abstract ( 207 )   PDF (3053KB) ( 202 )   Save
    For the characteristics of a high temperature and difficulty in heat dissipation of the winding end of a vehicle permanent magnet synchronous motor, this paper calculates the temperature rise characteristics of the motor under oil-water compound cooling conditions by adding an oil injection pipe to the end of the motor winding. The effects of water cooling and oil-water compound cooling on temperature rise characteristics of a permanent magnet synchronous motor are studied. The findings indicate that, when combined with an oil injection pipe at the winding end, the oil-water compound cooling strategy significantly cools the motor. The maximum temperature of the motor winding significantly reduces, and the temperature difference between high and low temperature zones is small, which plays a good role in temperature balancing. Besides, under the two rated operating conditions, compared with the water cooling scheme, the maximum temperature of the motor reduces by 27 ℃ in the oil-water compound cooling, with an improved cooling effect of 20% and 19% respectively.
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    Heat dissipation performance analysis and structure optimization of cooling channels for motors and controllers

    2023, 37 (2):  104-112.  doi: 10.3969/j.issn.1674-8425(z).2023.02.012
    Abstract ( 278 )   PDF (5079KB) ( 602 )   Save
    In order to reduce temperature rise of motor coils and controller IGBT, improve heat dissipation performance of the cooling channel for motors and controllers, and ensure that the cooling channel has low flow resistance, this paper takes the cooling channel structure of the driving system of a micro electric vehicle and a motor integrated with a controller as the research objects, and establishes a fluid-structure coupling analysis model of motors and controllers through CFD method. The internal velocity field, flow resistance characteristics, motor winding temperature field and IGBT temperature field under the original structure of the cooling channel are analyzed. The collaborative optimization method is used to intelligently optimize the structural parameters of the cooling channel for the motor and the controller, and seek the cooling channel structure with low flow resistance and efficient cooling performance. The results show that the original structure of the cooling channel has local vortexes and flow dead zones, the flow resistance of the cooling channel t is 31.87 kPa, and the maximum temperature of the motor winding is 120.04 ℃. Under the condition of meeting the goal of an optimization of manufacturing process and flow resistance, a structure with the most significant improvement of winding temperature rise is selected as the final optimization result. After optimization, local vortexes and flow dead zones of the cooling channel are improved, the flow is smoother, and the flow velocity is more uniform. The flow resistance decreases significantly, and the flow resistance decreases by about 12.5 kPa or 39.4% compared with the original model. The maximum temperature at the winding end of the optimized motor reduces by 3.28 ℃ or 2.7%. The research results can provide a theoretical reference for the design and improvement of the cooling structure of motors and controllers for new energy vehicles.
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    Research on the control strategy of the heat pump AC system for pure electric vehicles

    2023, 37 (2):  113-122.  doi: 10.3969/j.issn.1674-8425(z).2023.02.013
    Abstract ( 419 )   PDF (4816KB) ( 438 )   Save
    In order to maintain thermal comfort of the cabin environment of a pure electric vehicle and improve efficiency of energy use, this paper studies the control strategy of the heat pump air conditioning system for pure electric vehicles. AMESim is used to build a cabin simulation platform of the pure electric vehicle heat pump air conditioning system, and three control strategies are designed: compressor on/off control strategy, PID control strategy and fuzzy control strategy. Matlab/Simulink is used to simulate the three control strategies respectively, and then feedforward control is added and simulated on the basis of the fuzzy control strategy. The results show that the COP values under the compressor on/off control strategy, the PID control strategy and the fuzzy control strategy are about 1.5, 1.8 and 1.85 respectively; the temperature fluctuation is about 0.5 ℃ before the introduction of feedforward control, and there is no significant change after the introduction. The control performance of the fuzzy control strategy is better than that of the compressor on/off control and the PID control in terms of dynamic response and energy efficiency ratio of the system; after adding the feedforward control, the fuzzy control strategy effectively improves the dynamic response of the system when the external environment changes, and reduces the fluctuation of the cabin temperature.
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    Optimal design of battery direct cooling of pure electric vehicles under refrigeration conditions

    2023, 37 (2):  123-133.  doi: 10.3969/j.issn.1674-8425(z).2023.02.014
    Abstract ( 288 )   PDF (3933KB) ( 396 )   Save
    Aiming at the relatively independent air conditioning system, battery thermal management system and motor thermal management system of a pure electric vehicle, this paper optimizes an integrated thermal management system for battery direct cooling in summer. Firstly, a battery heat generation model is built based on the experiment, and the battery cooling mode in the battery heat management system changes from liquid cooling to more efficient refrigerant direct cooling. Then, an optimization model of one-dimensional vehicle heat management system is built and simulated under the new European driving cycle. The simulation results show that, after battery direct cooling, the vehicle thermal management system has better temperature control effect on the passenger compartment, battery and its temperature difference. Compared with the liquid cooling system, its refrigeration energy efficiency ratio increases by 0.47 and the vehicle electric consumption reduces by 3.1%.
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    Machinery and materials

    Thermoelastic coupling analysis of automobile wood ceramic brake linings

    2023, 37 (2):  134-141.  doi: 10.3969/j.issn.1674-8425(z).2023.02.015
    Abstract ( 173 )   PDF (3525KB) ( 123 )   Save
    In this paper, an automobile brake lining made of wood ceramic is proposed, and a 3D model of a disc brake of a wood ceramic brake lining is established to simulate the thermoelastic coupling characteristics of the disc brake under the action of the wood ceramic brake lining under various braking conditions. Based on the Archard wear model and the UMESHMOTION subroutine in the ABAQUS software, the numerical simulation of the wear depth of the wood ceramic brake lining is realized. The results show that, when wood ceramic is used as the friction material of automobile braking, the peak temperature of the brake disc during the braking process is 254.25 ℃, which is 9.91 ℃ lower than that of the traditional metal brake lining, and the stress distribution is uniform. It is beneficial to reduce the generation of thermal cracks in the brake disc, and at the same time reduce the influence of the “thermal decay” effect of the brake disc on the stability of the braking performance of the vehicle. The temperature of the wood ceramic brake lining can reach a maximum of 281.41 ℃ during the braking process, which is far lower than the thermal decomposition temperature of the wood ceramic material, and has good reliability. With wood ceramic as the friction material of the brake lining, the maximum wear depth of a single high-speed emergency braking does not exceed 2.11 μm, which has good wear resistance.
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    Effect of moulding process on curing deformation of the composite stiffened panel structure

    2023, 37 (2):  142-150.  doi: 10.3969/j.issn.1674-8425(z).2023.02.016
    Abstract ( 173 )   PDF (3623KB) ( 212 )   Save
    Considering the effect of different moulding processes, this paper studies the curing deformation of laminated composites of stiffened panel structures. The curing deformation is assessed after the stiffened panel structures of carbon fiber/epoxy resin composites are created through vacuum bag pressing process by co-curing, co-bonding, and secondary bonding and curing. For different moulding processes, by using an instantaneous linear elastic curing constitutive model and taking residual curing stresses as pre-stress, a finite element simulation method considering different curing methods is proposed, and is also compared and verified with the experimental results. The influences of factors such as moulding processes, layup methods and stiffener thickness on the curing deformation of the stiffened panel structures are analyzed. The results show that moulding processes, layup and stiffener thickness have a great influence on the curing deformation of the structures. The deformation of composite stiffened panel structures decreases considerably by increasing stiffener thickness and adopting an appropriate moulding process through a reasonable layup.
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    Gaussian process regression method for the frequency response function under impact loads

    2023, 37 (2):  151-157.  doi: 10.3969/j.issn.1674-8425(z).2023.02.017
    Abstract ( 152 )   PDF (2226KB) ( 195 )   Save
    A hammering test is an experimental modal analysis method widely used in academia and industry. The spectral estimation method is usually used to calculate the frequency response function (FRF), which is extremely susceptible to length and quality of the measurement data. In recent years, the Bayesian learning technology has provided a new exploration approach for the system and the control field. Inspired by this, the prior information of the FRF is statistically described by a complex Gaussian process, and a Bayesian inference method is developed for estimating the FRF under impact loads. The maximum posteriori estimation of the FRF is obtained, and the variance of the FRF estimation is also given. Furthermore, the numerical positive definiteness for optimizing the hyper-parameters is improved by applying QR decomposition. Finally, the effectiveness and reliability of the proposed method are verified by numerical examples and vibration experiments.
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    Multi-objective parameter optimization of EPB shield screw conveyors

    2023, 37 (2):  158-165.  doi: 10.3969/j.issn.1674-8425(z).2023.02.018
    Abstract ( 138 )   PDF (1750KB) ( 76 )   Save
    In order to improve transport efficiency, reduce the wear of EPB shield screw conveyors, and reduce power loss in the transport process, this paper proposes an optimization method for structure parameters of shield screw conveyors by combining discrete element software EDEM, NSGA-Ⅱ optimization algorithm and entropy weight TOPSIS method. Firstly, the discrete element software EDEM is simulated to obtain the performance indexes of mass flow rate, average spiral wear and operation power of a conveyor according to the designed orthogonal test, and the reliability of the simulation is verified. Secondly, Matlab software is used to fit the simulation data, and the performance index function between multiple structural parameters is established. Finally, NSGA-Ⅱ algorithm is used to optimize screw conveyor parameters, the Pareto frontier solution set is obtained, and the optimal solution is determined through entropy weight TOPSIS method. The results show that the overall performance of the conveyor is the best when the screw conveyor installation inclination angle is 24.69°, the pitch of the screw is 613.25 mm, the inner diameter of the screw is 696.34 mm, and the screw shaft diameter is 211.88 mm. Other conditions being the same, the mass flow rate increases by 10.56%, the screw shaft wear decreases by 8.64%, and the power loss reduces by 6.41%. The research results of this paper have a certain reference value for the design improvement of EPB screw conveyors.
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    A high precision absolute angular displacement sensor with a coprime number of measurement periods

    2023, 37 (2):  166-172.  doi: 10.3969/j.issn.1674-8425(z).2023.02.019
    Abstract ( 206 )   PDF (3048KB) ( 191 )   Save
    This paper proposes a high precision absolute time-grating angular displacement sensor with a coprime number of measurement periods. An incremental sensor with M measurement periods is added on the basis of a time-grating angular displacement sensor in the single-ring incremental electric field with N measurement periods so as to achieve combination measurement, among which two period numbers N and M of the incremental sensor are coprime numbers. The one with more measurement periods is used as the precision measurement component to improve the measurement accuracy, the one with fewer measurement periods is used as the rough measurement component, and the absolute angular displacement of the sensor is measured by using the corresponding relationship between the phase difference of the output traveling wave and the spatial angle of the two incremental sensors. The measurement accuracy is ensured by adopting a differential induction electrode structure to eliminate common-mode interference. This sensor with combined coprime numbers of measurement periods can not only easily realize absolute positioning, but also ensure a high accuracy of the sensor. A sensor prototype with an outer diameter of 154 mm, an inner diameter of 100 mm and a thickness of 2 mm is manufactured through the printed circuit board technology. The experimental platform of the sensor is built, and the prototype is tested. The test results show that the original accuracy of the sensor can reach ±1.8 in the whole circumference measurement range.
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    Information and computer science

    Fusion of MS3D-CNN and attention mechanism for hyperspectral image classification

    2023, 37 (2):  173-182.  doi: 10.3969/j.issn.1674-8425(z).2023.02.020
    Abstract ( 239 )   PDF (4768KB) ( 235 )   Save
    Aiming at the problems of insufficient utilization of spatial information and a shortage of sample labels in hyperspectral remote sensing image classification, this paper proposes an algorithm to classify hyperspectral images based on multi-scale 3D-CNN (MS3D-CNN) and Convolutional Block Attention Mechanism (CBAM). The feature mapping approach is employed to fully explore and fuse spatial and spectral features of hyperspectral images from different receptive fields, which are further processed by CBAM. After that, the deep neural network is constructed based on the idea of Residual Network (ResNet), and the Dropout method is encompassed to deal with the over-fitting problem. Finally, the processed features are classified by Softmax classifier. Extensive experiments are conducted on three hyperspectral datasets of Indian Pines, Pavia University and Salinas Valley, and the classification results show that the proposed method is superior to other classical methods.
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    Study on establishment and stability of the ecosystem evolutionary game model for online teaching platforms

    2023, 37 (2):  183-196.  doi: 10.3969/j.issn.1674-8425(z).2023.02.021
    Abstract ( 154 )   PDF (2105KB) ( 173 )   Save
    Taking online teaching and knowledge interaction as the research object, this paper studies the game interaction between relevant subjects on online teaching platforms and establishment and stability of online teaching platform ecosystem. For this, based on the idea of platform ecosystem, this paper establishes a three-way evolutionary game model involving knowledge providers, knowledge demanders and online teaching platforms, and reveals the operating mechanism of online teaching platform ecosystem. The results show that the differences of supply income, platform income and platform extra income have a positive impact on knowledge providers and strategy choice of online teaching platforms. The difference of cost has a negative impact on the strategy choice of participants, and also affects the strategy choice of other parties. The income influence coefficients of online teaching platform ecosystem participants mutually influence the strategic choice of other parties. The online teaching platform has a positive impact on income influence coefficient of the knowledge providers as well as strategy selection of online teaching platforms, and the income influence coefficient of deep learning has a positive impact on strategy selection of all of the three parties. With an increase of online teaching platform rewards, knowledge providers and online teaching platform strategies have evolved from participation and maintenance to deep participation and active development.
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    Research on IGBT failure prediction method combined with attention mechanism

    2023, 37 (2):  197-205.  doi: 10.3969/j.issn.1674-8425(z).2023.02.022
    Abstract ( 160 )   PDF (2825KB) ( 118 )   Save
    In recent years, the insulated gate bipolar transistor (IGBT) has been widely used in rail transportation, new energy sources and other fields. Its reliability research is currently a hot topic for scholars. Aiming at the reliability analysis of IGBT, this paper proposes a deep learning model based on long and short-term memory network and convolutional neural network (LSTM-CNN) as the backbone network for IGBT failure prediction. In the model, the introduced attention mechanism gives a higher weight of dominant factors to the features of different dimensions so as to strengthen the influence of important information. At the same time, cross-connection of the network structure fully extracts the features of different levels. The fused multi-level features improve the generalization and robustness of the model. This method is validated on IGBT accelerated aging dataset of National Aeronautics and Space Administration. The experimental results show that, compared with the current mainstream models, the root-mean-square error of the prediction accuracy of the attention mechanism and cross-connection improves by 1.27% and 0.78% respectively. Based on this, a network model based on the fusion of attention mechanism and LSTM-CNN with jump structures is further proposed, and its root-mean-square error of the prediction accuracy increases by 2.68%. It can be concluded that in the failure prediction of IGBT, attention mechanism and cross-connection improve the generalization and robustness of the model from different perspectives, which fully indicates the effectiveness of the proposed method.
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    Personnel dress detection method with human keypoints and attention mechanism

    2023, 37 (2):  206-214.  doi: 10.3969/j.issn.1674-8425(z).2023.02.023
    Abstract ( 210 )   PDF (2429KB) ( 206 )   Save
    In view of a lack of automatic supervision functions in industrial production safety systems, this paper designs a personnel dress detection algorithm for operators in construction sites, docks, mines and other construction sites, which is based on the localization of human keypoints and the attention mechanism of image areas to ensure the safety by carrying out the standard test for uniform wearing. The method innovatively combines the human pose estimation to locate the dress area, proposes an attention mechanism based on the image areas to effectively represent the features,and decouples the complex dress detection task into object detection and image classification tasks. The proposed approach improves the efficiency and performance of personnel dress detection in industrial scenarios. The experiments on the MSCOCO anddress detection datasets in the customizedcoal mine scene show that the proposed model achieves excellent results in personnel localization and dress detection tasks (AP50 is the best on the MSCOCO dataset). It is robust and can be applied to a variety of complex environments.
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    Research on parameter identification and error compensation algorithms of collaborative robots

    2023, 37 (2):  215-224.  doi: 10.3969/j.issn.1674-8425(z).2023.02.024
    Abstract ( 189 )   PDF (3708KB) ( 230 )   Save
    As intelligent operating assistants, collaborative robots have opened up a wide range of application scenarios in fields of industry, service and medical treatment. In order to explore the dynamic characteristics of collaborative robots and improve the accuracy of terminal positioning, this paper takes the KUKA LBR lightweight humanoid arm collaborative robot model as an example for research. Based on the RBF neural network and the synovial control algorithm, the dynamic control strategy of the collaborative robot is designed and the dynamic characteristics and end position error are analyzed. Parameter identification and error compensation of collaborative robots are carried out based on the Levenberg-Marquardt nonlinear damping least squares algorithm. The ADAMS-Matlab joint simulation shows that the dynamic control effect of a sliding mode controller based on the RBF neural network is better. The average terminal error under an extreme working condition is about 4.7 mm, which is mainly due to the influence of gravity load. After variable parameter error compensation, the average terminal error is less than 0.2 mm, which effectively improves the position accuracy and provides a theoretical basis for the research of collaborative robot control and error compensation.
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    Electrical and electronic

    Research on the energy extraction method for multistage transformer set ground wires with resonance compensation

    2023, 37 (2):  225-232.  doi: 10.3969/j.issn.1674-8425(z).2023.02.025
    Abstract ( 151 )   PDF (2984KB) ( 328 )   Save
    In order to solve the problem of power supply dead zones of an energy extraction device of a conventional current transformer under a weak current of an overhead wire, this paper proposes an energy extraction method for multistage transformer set ground wires with resonance compensation. Firstly, the principle of the method is analyzed, the equivalent circuit model is established, and the functional relationship between the related parameters and the current and output power of each circuit in the system is deduced. Secondly, reactive power compensation is carried out based on the matching capacitance of system parameters to achieve the maximum power output of the system. Then, a simulation circuit is designed to verify the feasibility of the proposed energy extraction method. Finally, an experimental device is built to verify the feasibility of the scheme. The experimental results show that, when the ground current is as low as 3 A, the output power of the device can reach 1.46 W, which is 9~10 times of the output power of the conventional resonant compensation method of the transformer, and meets the power supply requirements of online monitoring terminal equipment.
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    Research of state recognition and application of GPS-based bus operation

    2023, 37 (2):  233-240.  doi: 10.3969/j.issn.1674-8425(z).2023.02.026
    Abstract ( 125 )   PDF (3898KB) ( 228 )   Save
    By using the bus GPS data and map matching algorithm, this paper depicts the actual bus running status, counts the one-way time consumption and arrival time interval of the bus, quantitatively analyzes the reliability of bus travel time, evaluates the running effect of bus lines, and puts forward a method of identifying road sections with high traffic congestion based on an increasing rate of travel time. With the bus track data in Harbin as an example, this paper analyzes the operation of a bus No.84. The results show that the one-way punctuality of this line is 88.33%, and the one-way punctuality stability is 61.55%. The congestion model based on an increasing rate of travel time can effectively identify frequently congested sections of the line, and spatial distribution of the congested sections can be seen on the heat map. Through the analysis of the track data, changing characteristics of the bus operation are discovered, which provides an important reference for solving urban traffic congestion and improving bus service level and reliability.
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    Short-term prediction of wind power based on improved kernel extreme learning machines

    2023, 37 (2):  241-250.  doi: 10.3969/j.issn.1674-8425(z).2023.02.027
    Abstract ( 127 )   PDF (2904KB) ( 120 )   Save
    Aiming at the problem that wind power generation fluctuates greatly due to environmental changes and a kernel extreme learning machine is easy to fall into the local optimal solution, this paper constructs a short-term wind power prediction model of an optimized kernel extreme learning machine based on Complete Ensemble Empirical Mode Decomposition with Adaptive Noise (CEEMDAN) Analysis, wavelet threshold denoising and particle swarm algorithm. Firstly, CEEMDAN is used to decompose the environmental factors that are closely related to the output power of wind power generation, and several modal components with strong regularity are obtained. Besides, the threshold denoising method is used to denoise the first modal component containing much noise to weaken the non-stationarity of environmental factors. Then, after particle swarm optimization, the decomposed subcomponents and historical wind power data are used as the input of the kernel extreme learning machine algorithm for prediction. Finally, the measured data of a wind farm in Zhangjiakou, Hebei Province are selected for experimental comparison and analysis. The experimental results show that the improved wind power forecasting combination model proposed in this paper has higher forecasting accuracy and is suitable for wind power forecasting in different seasons.
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    Information and computer science

    Influence of haze parameters on external insulation characteristics of corona-aged silicone rubber

    2023, 37 (2):  251-259.  doi: 10.3969/j.issn.1674-8425(z).2023.02.028
    Abstract ( 133 )   PDF (4180KB) ( 141 )   Save
    In order to study the co-aging effect of various parameters of haze and corona discharge on composite insulator sheds, this paper uses a silicone rubber material as the test sample to carry out corona aging tests for different time periods in single haze, salt fog and haze environments. The flashover voltage, resistivity, hydrophobicity and leakage current of the aging test sample are evaluated and analyzed. The results show that the flashover voltage decreases with the increase of the aging time. The surface resistivity, volume resistivity and hydrophobic loss characteristics of the sample after aging have little to do with the aging environment. At the early stage of corona aging, the particle impact in the haze environment is the main reason for the destruction of the silicone rubber surface. At the later stage, the inorganic salt in the environment accelerates the corona aging, and the effect of ammonium sulfate is stronger than that of sodium nitrate. The research results can provide a theoretical basis for evaluating the service life of composite insulators in haze-prone areas and a reference for anti-haze and anti-pollution flashover of composite insulators.
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    Multi-time scale MPC energy management strategy for smart buildings with electric vehicles

    2023, 37 (2):  260-271.  doi: 10.3969/j.issn.1674-8425(z).2023.02.029
    Abstract ( 158 )   PDF (4551KB) ( 79 )   Save
    Considering travel characteristics of electric vehicles, this paper proposes a multi-time scale energy management strategy based on model predictive control for smart buildings with electric vehicles. Firstly, travel characteristics of electric vehicles are analyzed, and Monte Carlo is used to extract the arrival and departure time of electric vehicles, as well as the loading state at the beginning and end of charging. Secondly, the distributed power supply, energy storage and controllable load models of smart buildings are established, and the day-ahead energy management strategy is proposed based on mixed integer quadratic programming. Then, the intra-day rolling optimization strategy based on model predictive control is proposed to realize the dynamic correction of the day-ahead operation scheme. Finally, compared with a variety of energy management strategies, it is shown that the proposed strategy can effectively solve the problem of tie line power fluctuation caused by the day-ahead prediction error, improve the robustness of the system in the scenario of prediction uncertainty, reduce cost of the system, and improve the economy of the system.
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    Mathematics·Statistics

    Reliability evaluation model of multi-source heterogeneous data based on Bayesian variable weight fusion

    2023, 37 (2):  272-277.  doi: 10.3969/j.issn.1674-8425(z).2023.02.030
    Abstract ( 173 )   PDF (1260KB) ( 191 )   Save
    In order to solve the problem of a small number of sample data of products in reliability evaluation, this paper uses a Bayesian method to establish a reliability evaluation model for multi-source heterogeneous variable weight fusion. Firstly, this paper analyzes the tail gradient characteristics of the likelihood function of each information source and field data, and determines the weight of each information source through the absolute value error of the two. Then, considering the influence of the field test data on the weight of prior and multi-source fusion, this paper conducts reliability analysis of the product based on the Bayesian variable weight fusion method. Finally, the example shows that the variable weight fusion model is reasonable and effective, and the evaluation effect is better than the reliability evaluation model of Bayesian equal weight fusion.
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    Improvement of STSS model and its application

    2023, 37 (2):  278-288.  doi: 10.3969/j.issn.1674-8425(z).2023.02.031
    Abstract ( 136 )   PDF (4114KB) ( 117 )   Save
    With a rapid development of information technology and the advent of the era of big data, some spatio-temporal data sets are not only large-scale, but also contain various information variables. Therefore, appropriate expressions are needed to describe the characteristics of this kind of data. Based on the STSS model proposed by French and other scholars, the effects of covariate functions and periodic functions on response variables are introduced. The application of the STSS model on basis functions and penalty functions is used to smooth large-scale data. At the same time, it is accompanied by the influence of covariates and periodicity on the observed variables. This combination enables the improved model to describe the structure and changes of spatio-temporal data more comprehensively and realistically. Finally, superiority and applicability of the method are verified by using the simulated data and real spatio-temporal data.
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    Optimization model of the cold chain logistics distribution path of fresh agricultural products considering carbon emission

    2023, 37 (2):  289-297.  doi: 10.3969/j.issn.1674-8425(z).2023.02.032
    Abstract ( 372 )   PDF (1701KB) ( 333 )   Save
    Considering carbon emission, time windows, cargo loss and other factors, with the goal of minimizing distribution costs, this paper constructs an optimization model of the cold chain logistics distribution path of fresh agricultural products by calculating cost of carbon emission through carbon tax. Combined with the examples, the genetic algorithm is used to gain the solution, and the cold chain logistics path optimization scheme of fresh agricultural products is calculated with and without considering carbon emission respectively. The comparative analysis results show that considering carbon emission factors can effectively control and improve circulation rate of fresh agricultural products and reduce loss rate of fresh agricultural products. Logistic distribution costs are controlled and carbon emission is reduced, which provides effective decision-making for fresh supermarkets to optimize cold chain logistics and distribution.
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    “23rd International Conference of Fluid Power and Mechatronic Control Engineering” Special Column

    Characteristic analysis of flow field in an air atomizing nozzle

    2023, 37 (2):  298-306.  doi: 10.3969/j.issn.1674-8425(z).2023.02.033
    Abstract ( 141 )   PDF (4493KB) ( 84 )   Save
    This paper proposes a method to study the internal flow field characteristics of air atomizing nozzles based on a simulation software. The effects of different gas pressures and different liquid flows on the velocity, turbulent kinetic energy and liquid volume fraction of the flow field in the nozzle are analyzed by CFD simulation software. The results show that the velocity and turbulent kinetic energy hardly change with the change of gas pressure or liquid flow rate when the radial cross-sectional area of the mixing chamber is constant, and the liquid volume fraction keeps a uniform decreasing trend. After the radial sectional area of the mixing chamber reduces, the velocity and turbulent kinetic energy are positively correlated with the gas pressure when the liquid flow is constant, and the increasing amplitude is larger. The volume fraction of the liquid decreases with the increase of gas pressure, and the decreasing amplitude also increases constantly. The binding force on the liquid gradually increases with the increase of gas force. When the gas pressure is constant, the velocity and turbulent kinetic energy increase with the increase of the liquid flow, but the increasing amplitude gradually decreases. The liquid volume fraction decreases with the increase of the liquid flow, but the decreasing amplitude also gradually diminishes.
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    Fault diagnosis of planetary roller screw mechanism through one-class method

    2023, 37 (2):  307-315.  doi: 10.3969/j.issn.1674-8425(z).2023.02.034
    Abstract ( 142 )   PDF (3687KB) ( 105 )   Save
    Aiming at the problem that it is difficult to achieve fault decision-making due to an unknown failure mechanism of planetary roller screw mechanism (PRSM)and a shortage of faulty types of PRSM in practice,this paper proposes a one-class model called deep support vector data description (deep SVDD) to determine whether PRSM is normal or not. Firstly,vibration signals of PRSM are collected on a PRSM test bench under three working states, including normal state, failure of lubrication and failure of teeth on one side of the roller. Then, the data are normalized and enhanced by window cropping to expand the number of samples. After that,wavelet packet transform is used to initially extract features of the data by decomposing signals. Finally, deep SVDD is used to complete fault diagnosis of PRSM.Meanwhile, it is compared with one-class support vector machine (OCSVM) and support vector data description (SVDD). The results show that deep SVDD has a better classification ability and higher training efficiency, so it is suitable for fault diagnosis of PRSM.
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    Research on the fuzzy adaptive control method for SMA-driven finger rehabilitation robots

    2023, 37 (2):  316-323.  doi: 10.3969/j.issn.1674-8425(z).2023.02.035
    Abstract ( 137 )   PDF (3474KB) ( 255 )   Save
    Aiming at the problem that it is difficult to accurately control the bending angle of a finger rehabilitation robot driven by a shape memory alloy (SMA) wire, this paper proposes a fuzzy adaptive PID control algorithm. Based on the kinematics model and the alloy wire drive model of a finger rehabilitation robot, this method utilizes the bending angle feedback of resistance mapping and adopts fuzzy adaptive parameters to realize position control. An experimental test platform for finger rehabilitation robots is built, the traditional PID and fuzzy adaptive PID control methods are used to conduct position control experiments on the index finger, and position control effects of each index finger joint after stability are analyzed. The research results show that, compared with the traditional PID control algorithm, the fuzzy adaptive control algorithm takes 2~3 s longer to reach the angle steady state time, the adjustment time is shorter, the position error is 2°, and the adaptive adjustment can be completed within 3 s under external disturbance conditions. The proposed method has strong robustness and can better compensate the hysteresis in the SMA phase changing process, which proves its effectiveness.
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    Analysis of dynamic characteristics of rigid-flexible coupling of a small palletizing manipulator

    2023, 37 (2):  324-330.  doi: 10.3969/j.issn.1674-8425(z).2023.02.036
    Abstract ( 142 )   PDF (3114KB) ( 115 )   Save
    This paper designs a small one and conducts its kinematic study in three working positions. At present, the rigid-flexible coupling analysis of a manipulator is mostly the joint simulation of ANSYS software and ADAMS software, where a modal file of the flexible body is created in ANSYS software to use the bidirectional data exchange interface of ADAMS software to realize rigid-flexible coupling dynamics analysis. However, with continuous updating and iteration of the software, this analysis process can be realized in the transient dynamics analysis module Transient Structural configured in Workbench software, the simulation platform of ANSYS. SolidWorks software is used to design the model of the manipulator. Meanwhile, ANSYS software is used to simulate the statics and the rigid-flexible coupling dynamics of the manipulator. The experimental results show that the static rigidity analysis has a great influence on the accuracy of the results, and it is further concluded that the maximum equivalent stress analysis cannot accurately judge the safety of the structure. The simulation results of the rigid-flexible coupling show that, in three different positions, the maximum stress value of the large arm of the manipulator is 82.941 MPa, and the maximum moving speed of the large arm is 2.455 1 m/s. At the same time, the manipulator is also capable of reaching the maximum working range of 1 372.7 mm. The transient dynamics motion simulation by ANSYS can effectively predict motion patterns and stress distribution of the manipulator, and make further calculation of the service life of the manipulator.
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    Pharmaceutical·Biological Engineering

    Bibliometric analysis of the research trend of usnic acid in recent 30 years

    2023, 37 (2):  331-343.  doi: 10.3969/j.issn.1674-8425(z).2023.02.037
    Abstract ( 99 )   PDF (6524KB) ( 113 )   Save
    This paper carries out a bibliometric analysis of the literatures of usnic acid published in the past 30 years to discuss its current research status, hotspots and development trend. The relevant research literatures of usnic acid published on Web of Science from 1990 to 2021 are retrieved. Then, scientometrics tools like VOSviewer1.6.17 and CiteSpace5.8.R3 are used to create network knowledge maps of usnic acid, and co-occurrence and cluster analysis of countries, authors and keywords are done. Finally, a total of 998 foreign articles are included in the quantitative analysis. The annual publication volume of usnic acid-related research fields is increasing year by year, showing a trend of dynamic development, with a high research interest especially in the past 15 years. Research authors from 76 countries around the world have formed a number of close contact teams. China ranks the fifth in the number of foreign literatures published from 1990 to 2021, and has cooperated with 15 countries in usnic acid researches. In the past 30 years, scholars from all over the world have carried out comprehensive researches on usnic acid in terms of natural sources, extraction and separation, pharmacological activity and toxicity. In the past 5 years, compared with the early and mid-term research stages, the research hotspots of usnic acid have gradually shifted from sources, extraction and isolation, anti-inflammation and anti-bacteria, and antioxidant activities to anti-tumor activity evaluation and mechanism researches, and the research of new delivery systems transforms. A visualization method is used to analyze usnic acid related literatures, and the research status of usnic acid is displayed in the form of knowledge graphs, which can better predict the research frontier, hotspots and development trend of usnic acid, and provide reference and suggestions for the follow-up research.
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    Prokaryotic expression and identification of rabies virus nucleocapsid protein

    2023, 37 (2):  344-349.  doi: 10.3969/j.issn.1674-8425(z).2023.02.038
    Abstract ( 126 )   PDF (1628KB) ( 132 )   Save
    In order to realize efficient expression of nucleoprotein (N) from rabies virus (RV) in the prokaryotic system, this paper refers to the RV N gene sequence published by GenBank (accession number 1489853). Without changing the amino acid sequence of the RV N protein, the codon of the gene is optimized, and the N gene is chemically synthesized and cloned into the prokaryotic expression vector pET28a(+). The recombinant plasmid pET28a(+)/RV N is constructed and transformed into E. coli BL21 (DE3) competent cells for expression. The recombinant RV N protein is purified by His-tag nickel column. The purified RV N protein is then identified by SDS-PAGE and analyzed by Western blot. The results show that the recombinant plasmid is identified by double enzyme digestion, and a band appears at 1 359 bp, which is completely consistent with the corresponding known gene sequence after sequence tests. When the recombinant bacteria are induced in an IPTG concentration of 0.1 mmol/L at 30 ℃ for 6 hours, the RV N protein expression is the highest. The RV N recombinant protein is obtained through the purification of the nickel column. The concentration of the recombinant RV N protein measured by BCA method is 1.783 mg/mL. An obvious western blot appears at 51 kD, which is consistent with the expected target band. It shows that RV N recombinant protein is successfully expressed in E. coli BL21 (DE3) competent cells, which lays a foundation for the subsequent rabies detection and the development of a new vaccine.
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    Preparation of polyclonal antibodies against Locustamigratoria DSCAM2

    2023, 37 (2):  350-356.  doi: 10.3969/j.issn.1674-8425(z).2023.02.039
    Abstract ( 120 )   PDF (1815KB) ( 81 )   Save
    In this study,the prepared Down syndrome cell adhesion molecule 2 (DSCAM2) polyclonal antibodiesare intended to provide an immunological tool for functional exploration of DSCAM2. This project employs bioinformatics approaches to analyze the DSCAM2 antigenic structure, and constructs DSCAM2828-957recombinant expression strain by DNA recombination technology.The recombinant DSCAM2828-957 proteinis purified by Ni affinity chromatography and Anti-DSCAM2 polyclonal antibodiesare prepared by immunized mice. Antibody titers are detected by ELISA, antibody specificity is detected by Western Blot,and DSCAM2 localization in Locustamigratoria hemocytes is detected by Wright-Giemsa staining and immunofluorescence.DSCAM2 is knocked down by siRNA,and hemocytes are counted. The results show that the constructed pET30a(+)-DSCAM2828-957/BL21(DE3) prokaryotic strain can successfully express the recombinant DSCAM2828-957 protein.The immuned mice with DSCAM2828-957 recombinant antigens obtained through Ni chelating affinity chromatography gain polyclonal antibodies with high titer and good specificity.Cell localization analysis shows a high expression of DSCAM2 in basophils of Locustamigratoria.A knockdown of DSCAM2 expression level increases the number of total hemocytes in Locustamigratoria.
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    3D-QSAR study and optimized design of 1-deoxy-D-xylulose-5-phosphate reductoisomerase inhibitors of Mycobacterium tuberculosis

    2023, 37 (2):  357-368.  doi: 10.3969/j.issn.1674-8425(z).2023.02.040
    Abstract ( 142 )   PDF (2236KB) ( 100 )   Save
    Mycobacterium tuberculosis(Mtb) is a pathogen of tuberculosis, and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is a key rate-limiting enzyme in the metabolism of Mtb, so it is thought to be a potential antibacterial target. This paper collects 35 fosmidomycin derivatives with in vitro inhibitory activity against Mycobacterium tuberculosis1-deoxy-D-xylulose-5-phosphate reductoisomerase (mtDXR), conducts a three dimensional quantitative structure-activity relationship between them by using comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA), and establishes corresponding models. The results show that, for CoMFA model, the best principal component value n is 7, the cross validation coefficient q2 is 0.601,and the correlation coefficient r2 is 0.979. For CoMSIA model, the best principalcomponent value n is 8, the cross validation coefficient q2 is 0.609, and the correlation coefficient r2 is 0.983. The data show that both CoMFA and CoMSIA models have good predictability. Meanwhile, the molecular docking method is introduced to further investigate the non-bonding interaction between these small molecule inhibitors of fosmidomycin and amino acid residues on target active sites.Combined with contour maps of 3D-QSAR, the modification areas of molecular structure of these compounds are determined which can be processed and optimized.On the basis of these work, this paper designs 14 new fosmidomycin derivatives, predicts their activity, and obtains a new compound 27m with higher predictive activity. The conclusions derived from this paper provide a theoretical basis and important reference for the further development of new mtDXR inhibitors.
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