Abstract: In this study,the prepared Down syndrome cell adhesion molecule 2 (DSCAM2) polyclonal antibodiesare intended to provide an immunological tool for functional exploration of DSCAM2. This project employs bioinformatics approaches to analyze the DSCAM2 antigenic structure, and constructs DSCAM2828-957recombinant expression strain by DNA recombination technology.The recombinant DSCAM2828-957 proteinis purified by Ni affinity chromatography and Anti-DSCAM2 polyclonal antibodiesare prepared by immunized mice. Antibody titers are detected by ELISA, antibody specificity is detected by Western Blot,and DSCAM2 localization in Locustamigratoria hemocytes is detected by Wright-Giemsa staining and immunofluorescence.DSCAM2 is knocked down by siRNA,and hemocytes are counted. The results show that the constructed pET30a(+)-DSCAM2828-957/BL21(DE3) prokaryotic strain can successfully express the recombinant DSCAM2828-957 protein.The immuned mice with DSCAM2828-957 recombinant antigens obtained through Ni chelating affinity chromatography gain polyclonal antibodies with high titer and good specificity.Cell localization analysis shows a high expression of DSCAM2 in basophils of Locustamigratoria.A knockdown of DSCAM2 expression level increases the number of total hemocytes in Locustamigratoria.